NSJ Bioreagents
SKU:R31552
ATF2 Antibody / Activating Transcription Factor 2
ATF2 Antibody / Activating Transcription Factor 2
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Activating transcription factor 2 is a protein that in humans is encoded by the ATF2 gene. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. It binds to the cAMP-responsive element (CRE), an octameric palindrome. ATF2 forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus, it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB, FACS, IHC-P, IHC-F |
| Application Details | Western blot: 0.5-1ug/ml,FACS: 1-3ug/10^6 cells,IHC (FFPE): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml |
| Application Note | The stated application concentrations are suggested starting points. Titration of the ATF2 antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Localization | Nuclear, cytoplasmic |
| Immunogen | Human partial recombinant protein (AA 93-450) was used as the immunogen for this ATF2 antibody. |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide |
| Purity | Antigen affinity |
| Storage | After reconstitution, the ATF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This ATF2 antibody is available for research use only. |
| Uniprot # | P15336 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/atf2-antibody-activating-transcription-factor-2-r31552 |
| Title | ATF2 Antibody / Activating Transcription Factor 2 |
| Description | Activating transcription factor 2 is a protein that in humans is encoded by the ATF2 gene. This gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins. It binds to the cAMP-responsive element (CRE), an octameric palindrome. ATF2 forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro, thus, it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined. |
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