• IHC testing of FFPE human skeletal muscle tissue with Aspartoacylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F54432-0.08ML

Aspartoacylase Antibody / ASPA

Aspartoacylase Antibody / ASPA

$205.00 USD
$205.00 USD
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ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application IF, WB, FACS, IHC-P
Application Details Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the Aspartoacylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Nuclear, cytoplasmic
Immunogen A portion of amino acids 82-110 from the human protein was used as the immunogen for the Aspartoacylase antibody.
Purity SAS precipitation
Storage Aliquot the Aspartoacylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Aspartoacylase antibody is available for research use only.
Uniprot # P45381
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/aspartoacylase-antibody-aspa-f54432
Title Aspartoacylase Antibody / ASPA
Description ASPA is an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues.
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