• Immunofluorescent staining of FFPE human A431 cells with ASIC2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
1 1

NSJ Bioreagents

RQ6038

ASIC2 Antibody / ACCN1

ASIC2 Antibody / ACCN1

$449.00 USD
$449.00 USD
Sale Sold out
Shipping calculated at checkout.
Size

Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2,is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IF, FACS, IHC-P, Direct ELISA
Application Details Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note Optimal dilution of the ASIC2 antibody should be determined by the researcher.
Immunogen Recombinant human protein (amino acids H27-C512) was used as the immunogen for the ASIC2 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Affinity purified
Storage After reconstitution, the ASIC2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This ASIC2 antibody is available for research use only.
Uniprot # Q16515
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/asic2-antibody-accn1-rq6038
Title ASIC2 Antibody / ACCN1
Description Amiloride-sensitive cation channel 1, neuronal, also known as ASIC2,is a protein that in humans is encoded by the ACCN1 gene. This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene may play a role in neurotransmission. In addition, a heteromeric association between this member and acid-sensing (proton-gated) ion channel 3 has been observed to co-assemble into proton-gated channels sensitive to gadolinium. Alternative splicing has been observed at this locus and two variants, encoding distinct isoforms, have been identified.
View full details