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NSJ Bioreagents
SKU:F52059-0.05ML
Anti-His Antibody
Anti-His Antibody
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$205.00 USD
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Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Clone | 6AT18 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Application | WB, ELISA |
| Application Details | Western blot: 1:1000-1:4000 |
| Application Note | The stated application concentrations are suggested starting amounts. Titration of the anti-His antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal anti-His antibody. |
| Purity | Purified |
| Storage | Store at 4oC for up to one month. For long-term, aliquot the anti-His antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This anti-His antibody is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/anti-his-antibody-6at18-f52059 |
| Title | Anti-His Antibody |
| Description | Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18, provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. NSJBio's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells. |
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