• Western blot analysis of anti-HER2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ERBB2 gene (2).
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NSJ Bioreagents

F50601-0.08ML

Anti-HER2 Antibody

Anti-HER2 Antibody

$205.00 USD
$205.00 USD
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HER2/ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Predicted Species Reactivity Mouse, Rat
Application WB, IHC, ELISA
Application Details Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note Titration of the anti-HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 1227-1255 from the human protein was used as the immunogen for this anti-HER2 antibody.
Purity Antigen affinity
Storage Aliquot the anti-HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This anti-HER2 antibody is available for research use only.
Uniprot # P04626
Status Available
PDF Link http://www.nsjbio.com/tds-pdf/anti-her2-antibody-f50601
Title Anti-HER2 Antibody
Description HER2/ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.
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