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NSJ Bioreagents
SKU:F52068-0.08ML
Anti-GST Antibody
Anti-GST Antibody
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Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In 1X PBS, pH 7.4, with 0.09% sodium azide |
| Format | Purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Application | WB, ELISA |
| Application Details | Western blot: 1:1000 |
| Application Note | Titration of the anti-GST antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | Recombinant full length GST was used as the immunogen for this anti-GST antibody. |
| Purity | Purified |
| Storage | Aliquot the anti-GST antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. |
| Limitation | This anti-GST antibody is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/anti-gst-antibody-f52068 |
| Title | Anti-GST Antibody |
| Description | Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells. |
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