• Fluorescent image of U251 cells stained with anti-ATG5 antibody diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). DAPI was used as a nuclear counterstain (blue).
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NSJ Bioreagents

F46219-0.08ML

Anti-ATG5 Antibody

Anti-ATG5 Antibody

$205.00 USD
$205.00 USD
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Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, IHC, IF, ELISA
Application Details Immunofluorescence: 1:25,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note Titration of the anti-ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 209-238 from the human protein was used as the immunogen for this anti-ATG5 antibody.
Purity Antigen affinity
Storage Aliquot the anti-ATG5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This anti-ATG5 antibody is available for research use only.
Uniprot # Q9H1Y0
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/anti-atg5-antibody-f46219
Title Anti-ATG5 Antibody
Description Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
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