• Immunofluorescent staining of FFPE human A431 cells with Annexin VII antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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NSJ Bioreagents

R31187

Annexin VII Antibody

Annexin VII Antibody

$449.00 USD
$449.00 USD
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Annexin A7, also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al.(1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al.(2001) concluded that the gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al.(1996) present experimental evidence, based on studies of recombinant human protein and isolated bovine chromaffin cells, that ANXA7 is a Ca(2+)-dependent GTP binding protein. It was active in a chromaffin granule aggregation assays in the presence of Ca(2+) and GTP, and was deactivated upon GTP hydrolysis.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human
Application WB, IHC-P, IF, FACS
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence: 2-5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note The stated application concentrations are suggested starting amounts. Titration of the Annexin VII antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen An amino acid sequence from the C-terminus of human Annexin VII (TRSEIDLVQIKQMFAQ) was used as the immunogen for this Annexin VII antibody.
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Purity Antigen affinity
Storage After reconstitution, the Annexin VII antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Annexin VII antibody is available for research use only.
Uniprot # P20073
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/annexin-vii-antibody-r31187
Title Annexin VII Antibody
Description Annexin A7, also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al.(1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al.(2001) concluded that the gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al.(1996) present experimental evidence, based on studies of recombinant human protein and isolated bovine chromaffin cells, that ANXA7 is a Ca(2+)-dependent GTP binding protein. It was active in a chromaffin granule aggregation assays in the presence of Ca(2+) and GTP, and was deactivated upon GTP hydrolysis.
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