• Western blot testing of 1) human SiHa, 2) human HeLa, 3) human RT4, 4) human A549, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with AMPK alpha 1 antibody. Predicted molecular weight ~63 kDa.
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NSJ Bioreagents

RQ4362

AMPK alpha 1 Antibody / PRKAA1

AMPK alpha 1 Antibody / PRKAA1

$449.00 USD
$449.00 USD
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5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, ELISA
Application Details Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the AMPK alpha 1 antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen A recombinant human protein corresponding to amino acids F377-R446 was used as the immunogen for the AMPK alpha 1 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity purified
Storage After reconstitution, the AMPK alpha 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This AMPK alpha 1 antibody is available for research use only.
Uniprot # Q13131
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/ampk-alpha-1-antibody-prkaa1-rq4362
Title AMPK alpha 1 Antibody / PRKAA1
Description 5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme that in humans is encoded by the PRKAA1 gene. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
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