• Western blot testing of human 1) HepG2 and 2) JAr cell lysate with ALPL antibody. Predicted molecular weight ~57 kDa, but can be observed at up to ~75 kDa due to glycosylation.
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NSJ Bioreagents

RQ5119

ALPL Antibody / Alkaline Phosphatase

ALPL Antibody / Alkaline Phosphatase

$449.00 USD
$449.00 USD
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This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. [RefSeq]

Specifications

Family Primary antibody
Formulation Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Format Purified
Clone DBF-1
Host Animal Rabbit
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Species Reactivity Human
Application IHC-P, WB
Application Details Western blot: 1:5000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note Optimal dilution of the ALPL antibody should be determined by the researcher.
Immunogen A synthetic peptide specific to human Alkaline Phosphatase / ALPL was used as the immunogen for the ALPL antibody.
Purity Affinity purified
Storage Store the ALPL antibody at -20oC.
Limitation This ALPL antibody is available for research use only.
Uniprot # P05186
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/alpl-antibody-alkaline-phosphatase-dbf-1-rq5119
Title ALPL Antibody / Alkaline Phosphatase
Description This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. [RefSeq]
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