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NSJ Bioreagents

SKU:F54785-0.08ML

Alpha Enolase Antibody / ENOA

Alpha Enolase Antibody / ENOA

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ENOA, or ENO1, is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Application WB, IHC-P, IF, FACS
Application Details Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells)
Application Note The stated application concentrations are suggested starting points. Titration of the Alpha Enolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic, nuclear
Immunogen A portion of amino acids 405-434 from the human protein was used as the immunogen for the Alpha Enolase antibody.
Purity Purified
Storage Aliquot the Alpha Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Alpha Enolase antibody is available for research use only.
Uniprot # P06733
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/alpha-enolase-antibody-enoa-f54785
Title Alpha Enolase Antibody / ENOA
Description ENOA, or ENO1, is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
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