NSJ Bioreagents
SKU:RQ4994
Alpha B Crystallin Antibody / CRYAB
Alpha B Crystallin Antibody / CRYAB
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Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [RefSeq]
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA |
| Format | Purified |
| Clone | ABHC-3 |
| Host Animal | Rabbit |
| Clonality | Rabbit Monoclonal |
| Isotype | Rabbit IgG |
| Species Reactivity | Human |
| Application | WB |
| Application Details | Western blot: 1:500-1:2000 |
| Application Note | Optimal dilution of the Alpha B Crystallin antibody should be determined by the researcher. |
| Immunogen | A synthetic peptide specific to human CRYAB / Alpha B Crystallin was used as the immunogen for the Alpha B Crystallin antibody. |
| Purity | Affinity purified |
| Storage | Store the Alpha B Crystallin antibody at -20oC. |
| Limitation | This Alpha B Crystallin antibody is available for research use only. |
| Uniprot # | P02511 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/alpha-b-crystallin-antibody-cryab-abhc-3-rq4994 |
| Title | Alpha B Crystallin Antibody / CRYAB |
| Description | Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [RefSeq] |
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