• Western blot testing of 1) human SH-SY5Y, 2) human U-87 MG, 3) human MCF7, 4) human 293T, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with Alpha-Adaptin antibody. Predicted molecular weight ~108 kDa.
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NSJ Bioreagents

R30093

Alpha-Adaptin Antibody / AP2A1

Alpha-Adaptin Antibody / AP2A1

$449.00 USD
$449.00 USD
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Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.

Specifications

Family Primary antibody
Formulation 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Format Ascites
Clone 100/2
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Species Reactivity Human, Mouse, Rat
Application WB
Application Details Western blot: 1-2ug/ml
Application Note The stated application concentrations are suggested starting amounts. Titration of the Alpha-Adaptin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen AP-2 adaptor polypeptides from bovine brain was used as the immunogen for this Alpha-Adaptin antibody.
Purity Ascites
Storage After reconstitution, the Alpha-Adaptin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This Alpha-Adaptin antibody is available for research use only.
Uniprot # O95782
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/alpha-adaptin-antibody-ap2a1-1002-r30093
Title Alpha-Adaptin Antibody / AP2A1
Description Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1(HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC)and alpha-adaptin A and C. Coat proteins of approximately 100-kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels.
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