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NSJ Bioreagents
SKU:R30092
Alpha-Actinin Antibody
Alpha-Actinin Antibody
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Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS |
| Format | Ascites |
| Clone | EA-53 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1 |
| Species Reactivity | Human, Mouse, Rat, Rabbit |
| Application | WB, IHC-P, IHC-F |
| Application Details | Western blot: 0.5-1ug/ml,IHC (FFPE): 1.5-3ug/ml,IHC (Frozen): 1.5-3ug/ml to detect in formalin or acetone fixed tissues |
| Application Note | The stated application concentrations are suggested starting amounts. Titration of the alpha-Actinin antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody. |
| Purity | Ascites |
| Storage | After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This alpha-Actinin antibody is available for research use only. |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/alpha-actinin-antibody-ea-53-r30092 |
| Title | Alpha-Actinin Antibody |
| Description | Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin. |
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