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NSJ Bioreagents

SKU:F40345-0.08ML

Alkaline Phosphatase Antibody (placental)

Alkaline Phosphatase Antibody (placental)

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There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Placental Alkaline Phosphatase (ALPP) is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Clone 421CT8.1.1
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Species Reactivity Human
Application WB, ELISA
Application Details Western blot: 1:100-1:500
Application Note Titration of the Alkaline Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 56-83 from human ALPP was used as the immunogen for this Alkaline Phosphatase antibody.
Purity Purified
Storage Aliquot the Alkaline Phosphatase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Alkaline Phosphatase antibody is available for research use only.
Uniprot # P05187
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/alkaline-phosphatase-antibody-placental-421ct811-f40345
Title Alkaline Phosphatase Antibody (placental)
Description There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. Placental Alkaline Phosphatase (ALPP) is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
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