• Western blot analysis of lysate from 1) 293, 2) RD, 3) mouse NIH3T3 and 4) rat L6 cell line using Aldolase antibody at 1:1000. Predicted molecular weight ~40 kDa.
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NSJ Bioreagents

F48149-0.08ML

Aldolase Antibody

Aldolase Antibody

$205.00 USD
$205.00 USD
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Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse, Rat
Predicted Species Reactivity Rabbit
Application IF, IHC, WB, ELISA
Application Details Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note Titration of the Aldolase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 66-95 from the human protein was used as the immunogen for this Aldolase antibody.
Purity Purified
Storage Aliquot the Aldolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Aldolase antibody is available for research use only.
Uniprot # P04075
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/aldolase-antibody-f48149
Title Aldolase Antibody
Description Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
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