• Western blot analysis of lysate from 1) MCF-7, 2) HeLa cell line and 3) human skeletal muscle tissue lysate using ALDOA antibody at 1:1000. Predicted molecular weight ~40 kDa.
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NSJ Bioreagents

F48150-0.08ML

ALDOA Antibody

ALDOA Antibody

$205.00 USD
$205.00 USD
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Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human, Mouse
Predicted Species Reactivity Rabbit
Application WB, IHC, ELISA
Application Details Western blot: 1:1000,IHC (Paraffin): 1:10-1:50
Application Note Titration of the ALDOA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen A portion of amino acids 303-331 from the human protein was used as the immunogen for this ALDOA antibody.
Purity Purified
Storage Aliquot the ALDOA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This ALDOA antibody is available for research use only.
Uniprot # P04075
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/aldoa-antibody-f48150
Title ALDOA Antibody
Description Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
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