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NSJ Bioreagents
SKU:R32504
AKR1C1/2 Antibody
AKR1C1/2 Antibody
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AKR1C1 and AKR1C2 are members of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. AKR1C1 binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. The AKR1C1 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | 0.5mg/ml if reconstituted with 0.2ml sterile DI water |
| Format | Antigen affinity purified |
| Host Animal | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Species Reactivity | Human, Mouse, Rat |
| Application | WB, IF, FACS |
| Application Details | Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells |
| Application Note | Differences in protocols and secondary/substrate sensitivity may require the AKR1C1/2 antibody to be titrated for optimal performance. |
| Localization | Cytoplasmic |
| Immunogen | Amino acids M1-K123 from human AKR1C1 were used as the immunogen for the AKR1C1/2 antibody. |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| Purity | Antigen affinity |
| Storage | After reconstitution, the AKR1C1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing. |
| Limitation | This AKR1C1/2 antibody is available for research use only. |
| Uniprot # | Q04828, P52895 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/akr1c12-antibody-r32504 |
| Title | AKR1C1/2 Antibody |
| Description | AKR1C1 and AKR1C2 are members of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. AKR1C1 binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. The AKR1C1 gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. |
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