NSJ Bioreagents
SKU:F54034-0.1ML
AIF Antibody / AIFM1
AIF Antibody / AIFM1
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Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]
Specifications
| Family | Primary antibody |
|---|---|
| Formulation | In PBS with 50% glycerol and 0.03% ProClin 300 |
| Format | Purified |
| Clone | 8H1-B10-A12 |
| Host Animal | Mouse |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG2a |
| Species Reactivity | Human |
| Application | WB, ICC/IF |
| Application Details | Western blot: 1:1000,ICC/IF: 1:200 |
| Application Note | The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity. |
| Immunogen | A human recombinant partial protein was used as the immunogen for this AIF antibody. |
| Purity | Protein G affinity |
| Storage | Store the AIF antibody at -20oC. |
| Limitation | This AIF antibody is available for research use only. |
| Uniprot # | O95831 |
| Status | Available |
| PDF Link | https://www.nsjbio.com/tds-pdf/aif-antibody-aifm1-8h1-b10-a12-f54034 |
| Title | AIF Antibody / AIFM1 |
| Description | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt] |
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