• IHC testing of FFPE human brain tissue with AIF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F54468-0.08ML

AIF Antibody / AIFM1 / Apoptosis-Inducing Factor

AIF Antibody / AIFM1 / Apoptosis-Inducing Factor

$205.00 USD
$205.00 USD
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AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application FACS, IHC-P, WB, IF
Application Details Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000,Immunofluorescence: 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic, nuclear
Immunogen A portion of amino acids 70-98 from the human protein was used as the immunogen for the AIF antibody.
Purity Antigen affinity purified
Storage Aliquot the AIF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This AIF antibody is available for research use only.
Uniprot # O95831
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/aif-antibody-aifm1-apoptosis-inducing-factor-f54468
Title AIF Antibody / AIFM1 / Apoptosis-Inducing Factor
Description AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
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