• Immunofluorescent staining of FFPE human U-2 OS cells with AES antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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NSJ Bioreagents

RQ4533

AES Antibody / Amino-terminal enhancer of split

AES Antibody / Amino-terminal enhancer of split

$449.00 USD
$449.00 USD
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The AES gene is mapped to 19p13.3. The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human
Application WB, IF, FACS, Direct ELISA
Application Details Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the AES antibody should be determined by the researcher.
Immunogen Amino acids Y69-L138 from the human protein were used as the immunogen for the AES antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Antigen affinity purified
Storage After reconstitution, the AES antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This AES antibody is available for research use only.
Uniprot # Q08117
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/aes-antibody-amino-terminal-enhancer-of-split-rq4533
Title AES Antibody / Amino-terminal enhancer of split
Description The AES gene is mapped to 19p13.3. The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.
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