• IHC testing of FFPE human hepatocellular carcinoma tissue with Adenine phosphoribosyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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NSJ Bioreagents

F54787-0.08ML

Adenine phosphoribosyltransferase Antibody / APRT

Adenine phosphoribosyltransferase Antibody / APRT

$205.00 USD
$205.00 USD
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A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

Specifications

Family Primary antibody
Formulation In 1X PBS, pH 7.4, with 0.09% sodium azide
Format Purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Species Reactivity Human
Application WB, IF, FACS, IHC-P
Application Details Western blot: 1:500-1:1000, Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25
Application Note The stated application concentrations are suggested starting points. Titration of the Adenine phosphoribosyltransferase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cytoplasmic, nuclear
Immunogen A portion of amino acids 12-40 from the human protein was used as the immunogen for the Adenine phosphoribosyltransferase antibody.
Purity Antigen affinity purified
Storage Aliquot the Adenine phosphoribosyltransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitation This Adenine phosphoribosyltransferase antibody is available for research use only.
Uniprot # P07741
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/adenine-phosphoribosyltransferase-antibody-aprt-f54787
Title Adenine phosphoribosyltransferase Antibody / APRT
Description A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
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