• Western blot testing of 1) rat skeletal muscle, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse skeletal muscle, 6) mouse liver, 7) mouse lung and 8) mouse NIH 3T3 cell lysate with ACTN4 antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.
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NSJ Bioreagents

R32459

ACTN4 Antibody / Alpha Actinin 4

ACTN4 Antibody / Alpha Actinin 4

$449.00 USD
$449.00 USD
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Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P, IF, FACS
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note Optimal dilution of the ACTN4 antibody should be determined by the researcher.
Localization Cytoplasmic
Immunogen Amino acids E561-V661 from the human protein were used as the immunogen for the ACTN4 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity
Storage Prior to reconstitution, store at 4oC. After reconstitution, the ACTN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This ACTN4 antibody is available for research use only.
Uniprot # O43707
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/actn4-antibody-alpha-actinin-4-r32459
Title ACTN4 Antibody / Alpha Actinin 4
Description Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
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