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NSJ Bioreagents

SKU:RQ6228

ACTA1 Antibody

ACTA1 Antibody

Regular price $449.00 USD
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Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Clone 3H5
Host Animal Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1
Species Reactivity Human, Mouse, Rat
Application WB, IHC-P
Application Details Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note Optimal dilution of the ACTA1 antibody should be determined by the researcher.
Immunogen Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY from the human protein were used as the immunogen for the ACTA1 antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Affinity purified
Storage After reconstitution, the ACTA1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This ACTA1 antibody is available for research use only.
Uniprot # P68133
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/acta1-antibody-3h5-rq6228
Title ACTA1 Antibody
Description Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
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