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NSJ Bioreagents

SKU:R30731

5HT1A Receptor Antibody

5HT1A Receptor Antibody

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5HT1AR is a subtype of 5HTR that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. The HTR1A gene is located at 5q12.3. The decreases in 5HT1AR densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5HT1ARs has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. In the dorsal raphe nucleus they are co-localized with neurokinin 1(NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Rat
Application WB, IHC-P
Application Details Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml
Application Note The stated application concentrations are suggested starting amounts. Titration of the 5HT1A Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Localization Cell membrane
Immunogen An amino acid sequence from the C-terminus of human 5HT1A Receptor(NKDFQNAFKKIIKCKFCRQ) was used as the immunogen for this 5HT1A Receptor antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity
Storage After reconstitution, the 5HT1A Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This 5HT1A Receptor antibody is available for research use only.
Uniprot # P08908
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/5ht1a-receptor-antibody-r30731
Title 5HT1A Receptor Antibody
Description 5HT1AR is a subtype of 5HTR that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. The HTR1A gene is located at 5q12.3. The decreases in 5HT1AR densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5HT1ARs has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. In the dorsal raphe nucleus they are co-localized with neurokinin 1(NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.
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