• Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
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NSJ Bioreagents

RQ7948

4E-T Antibody / EIF4ENIF1

4E-T Antibody / EIF4ENIF1

$449.00 USD
$449.00 USD
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Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, FACS, IF, Direct ELISA
Application Details Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence: 5ug/ml
Application Note Optimal dilution of the 4E-T antibody should be determined by the researcher.
Immunogen E. coli-derived recombinant human protein (amino acids D13-Q985) was used as the immunogen for the 4E-T antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose
Purity Antigen affinity purified
Storage After reconstitution, the 4E-T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This 4E-T antibody is available for research use only.
Uniprot # Q9NRA8
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/4e-t-antibody-eif4enif1-rq7948
Title 4E-T Antibody / EIF4ENIF1
Description Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
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