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NSJ Bioreagents

SKU:RQ5931

14-3-3 gamma Antibody / YWHAG

14-3-3 gamma Antibody / YWHAG

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This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Specifications

Family Primary antibody
Formulation 0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format Antigen affinity purified
Host Animal Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Species Reactivity Human, Mouse, Rat
Application WB, FACS, Direct ELISA
Application Details Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note Optimal dilution of the 14-3-3 gamma antibody should be determined by the researcher.
Immunogen Recombinant human protein (amino acids Y107-A138) was used as the immunogen for the 14-3-3 gamma antibody.
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Purity Affinity purified
Storage After reconstitution, the 14-3-3 gamma antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitation This 14-3-3 gamma antibody is available for research use only.
Uniprot # P61981
Status Available
PDF Link https://www.nsjbio.com/tds-pdf/14-3-3-gamma-antibody-ywhag-rq5931
Title 14-3-3 gamma Antibody / YWHAG
Description This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
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