Nlrp3 Antibody

Size

100ug

Clone Number

Aliases

Nlrp3 antibody; Cias1 antibody; Mmig1 antibody; Nalp3 antibody; Pypaf1 antibody; NACHT antibody; LRR and PYD domains-containing protein 3 antibody; Cold autoinflammatory syndrome 1 protein homolog antibody; Cryopyrin antibody; Mast cell maturation-associated-inducible protein 1 antibody; PYRIN-containing APAF1-like protein 1 antibody

Immunogen Species

Mus musculus (Mouse)

UniProt ID

Q8R4B8

Immunogen

Recombinant Mouse NACHT, LRR and PYD domains-containing protein 3 protein (1-153AA)

Raised in

Rabbit

Species Reactivity

Mouse

Tested Applications

ELISA, IF; Recommended dilution: IF:1:50-1:200

Background

As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, beta-amyloid fibers, environmental or industrial particles and nanoparticles, etc. However, it is unclear what constitutes the direct NLRP3 activator. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5\'-GRRGGNRGAG-3\'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed:26098997).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

Nlrp3

Research Areas

Cancer; Cardiovascular; Immunology; Signal transduction