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SKU:PAA667Ra01
Polyclonal Antibody to Glucokinase (GCK)
Polyclonal Antibody to Glucokinase (GCK)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA667Ra01
Organism Species
Rattus norvegicus (Rat)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GCK. It has been selected for its ability to recognize GCK in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Life Sciences | Vitamin C and E chronic supplementation differentially affect hepatic insulin signaling in rats Pubmed:29291420 |
| Food & Function | Hypoglycemic effects of wheat bran alkyresorcinols in high-fat/high-sucrose diet and low-dose streptozotocin-induced type 2 diabetic male mice and protection of … Pubmed: 31094396 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA667Ra01 | Recombinant Glucokinase (GCK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA667Ra01 | Polyclonal Antibody to Glucokinase (GCK) | WB; IHC; ICC; IP. |
| MAA667Ra21 | Monoclonal Antibody to Glucokinase (GCK) | WB; IHC; ICC; IP. |
| SEA667Ra | ELISA Kit for Glucokinase (GCK) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA667Ra | Magnetic Luminex Assay Kit for Glucokinase (GCK) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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