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SKU:PAA260Ra01

Polyclonal Antibody to Beta-2-Microglobulin (b2M)

Polyclonal Antibody to Beta-2-Microglobulin (b2M)

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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAA260Ra01

Organism Species

Rattus norvegicus (Rat) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA260Ra01-Recombinant Beta-2-Microglobulin (b2M)

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against b2M. It has been selected for its ability to recognize b2M in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
BioMed Research International The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary Studies Hindawi: 740105
BMC Nephrology Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery Biomedcentral: Source
Nature Communications Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. pubmed:28959042
PLoS One Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis … Pubmed:30024955
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472
Toxicology Mechanisms and Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats Pubmed: 30426860
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Journal of Proteomics iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma 33540066
Antioxidants Diminishment of Nrf2 Antioxidative Defense Aggravates Nephrotoxicity of Melamine and Oxalate Coexposure. Antioxidants 2021, 10, 1464 34573096
Int Arch Occup Environ Health Biomarkers of cadmium exposure and renal function in estuarine adult villagers 34773507
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