Cloud-clone
SKU:PAA672Ra01
Polyclonal Antibody to Slit Homolog 2 (Slit2)
Polyclonal Antibody to Slit Homolog 2 (Slit2)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA672Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against Slit2. It has been selected for its ability to recognize Slit2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cancer Research | SLIT2 Attenuation during Lung Cancer Progression Deregulates β-Catenin and E-Cadherin and Associates with Poor Prognosis AACR: 702543 |
| Carcinogenesis | Slit-2 facilitates interaction of P-cadherin with Robo-3 and inhibits cell migration in an oral squamous cell carcinoma cell line OxfordJournals: source |
| PLoS One | Analytical Performance of ELISA Assays in Urine: One More Bottleneck towards Biomarker Validation and Clinical Implementation Pubmed:26889680 |
| ESC Heart Fail | Crosstalk between the activated Slit2¨CRobo1 pathway and TGF©\¦Â1 signalling promotes cardiac fibrosis 33236535 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| APA672Ra01 | Active Slit Homolog 2 (Slit2) | Cell culture; Activity Assays. |
| RPA672Ra01 | Recombinant Slit Homolog 2 (Slit2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA672Ra01 | Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
| LAA672Ra71 | Biotin-Linked Polyclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC. |
| MAA672Ra21 | Monoclonal Antibody to Slit Homolog 2 (Slit2) | WB; IHC; ICC; IP. |
| SEA672Ra | ELISA Kit for Slit Homolog 2 (Slit2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA672Ra | Magnetic Luminex Assay Kit for Slit Homolog 2 (Slit2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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