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SKU:PAA792Hu01
Polyclonal Antibody to Topoisomerase II (TOP2)
Polyclonal Antibody to Topoisomerase II (TOP2)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA792Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA792Hu01-Recombinant Topoisomerase II (TOP2)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TOP2. It has been selected for its ability to recognize TOP2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319 |
| Bioorganic & Medicinal Chemistry | Synthesis of pharmacologically important naphthoquinones and anticancer activity of 2-benzyllawsone through DNA topoisomerase-II inhibition. pubmed:28094224 |
| European Journal of Medicinal Chemistry | Antiproliferative activity of diarylnaphthylpyrrolidine derivative via dual target inhibition Pubmed: 31884407 |
| Int J Mol Sci | 2-{5-[(Z,2Z)-2-Chloro-3-(4-nitrophenyl)-2-propenylidene]-4-oxo-2-thioxothiazolidin-3-yl}-3-methylbutanoic Acid as a Potential Anti-Breast Cancer Molecule Pubmed:35456915 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA792Hu01 | Recombinant Topoisomerase II (TOP2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA792Hu01 | Polyclonal Antibody to Topoisomerase II (TOP2) | WB; IHC; ICC; IP. |
| MAA792Hu22 | Monoclonal Antibody to Topoisomerase II (TOP2) | WB; IHC; ICC; IP. |
| SEA792Hu | ELISA Kit for Topoisomerase II (TOP2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA792Hu | Magnetic Luminex Assay Kit for Topoisomerase II (TOP2) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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