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SKU:MAA930Hu22

Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)

Monoclonal Antibody to Extracellular Signal Regulated Kinase 2 (ERK2)

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UOM
Concentration
Organism species
Western blotting: 0.2-1ug/ml;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

MAA930Hu22

Organism Species

Homo sapiens (Human).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

C1

Ig Isotype

IgG2a Kappa

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPA930Hu02-Recombinant Extracellular Signal Regulated Kinase 2 (ERK2)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Chicken (Gallus)

Applications

WB; IHC

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a mouse monoclonal antibody raised against ERK2. It has been selected for its ability to recognize ERK2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/ml;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Cellular and Molecular Neurobiology Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease Pubmed:26119304
Graefe's Archive for Clinical and Experimental Ophthalmology Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909
International Journal of Reproductive Medicine Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317
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