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SKU:PAA998Hu01

Polyclonal Antibody to Glypican 4 (GPC4)

Polyclonal Antibody to Glypican 4 (GPC4)

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UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

PAA998Hu01

Organism Species

Homo sapiens (Human) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA998Hu01-Recombinant Glypican 4 (GPC4)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml



SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
PLoS One. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation Pubmed:Pmc4072672
Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions. 250690_2_G5_Svajunas_S.pdf
Intensive Care Medicine Experimental Elevated plasma glypicans are associated with organ failure in patients with infection Pubmed: 30618011
Crit Care Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgery Pubmed: 31138247
J Endocrinol Invest Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247
Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
Sci Rep Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status 34903856
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA998Hu01 Recombinant Glypican 4 (GPC4) Positive Control; Immunogen; SDS-PAGE; WB.
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LAA998Hu71 Biotin-Linked Polyclonal Antibody to Glypican 4 (GPC4) WB; IHC; ICC.
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