Cloud-clone
SKU:MAB040Hu22
Monoclonal Antibody to Vimentin (VIM)
Monoclonal Antibody to Vimentin (VIM)
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;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAB040Hu22
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C12
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB040Hu01-Recombinant Vimentin (VIM)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
Applications
WB; IHC; IF
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-40µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cancer Epidemiology, Biomarkers & Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer pubmed:22315367 |
| Scientific Reports | Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. pubmed:27898103 |
| Journal of Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin Pubmed:29477555 |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328 |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts Pubmed: 31729155 |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? Pubmed: 32088750 |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221 |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996 |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB040Hu02 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB040Hu01 | Active Vimentin (VIM) | Cell culture; Activity Assays. |
| RPB040Hu01 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB040Hu02 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| PAB040Hu01 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| LAB040Hu71 | Biotin-Linked Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC. |
| MAB040Hu24 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC |
| MAB040Hu25 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| MAB040Hu21 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu23 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu27 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu22 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| SEB040Hu | ELISA Kit for Vimentin (VIM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB040Hu | Magnetic Luminex Assay Kit for Vimentin (VIM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB040Hu01 | ELISA Kit DIY Materials for Vimentin (VIM) | Main materials for "Do It (ELISA Kit) Yourself". |
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