Cloud-clone
SKU:PAB091Ra01
Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL)
Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
PAB091Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB091Ra01-Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ALPL. It has been selected for its ability to recognize ALPL in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Materials Science: Materials in Medicine | Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451 |
| Osteoporosis International | Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250 |
| Journal of Applied Toxicology | Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source |
| Acta Pharmacologica Sinica | The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359 |
| Biomedical Materials | Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067 |
| Pharmacology | Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861 |
| J Biomed Mater Res A. | New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050 |
| J Bone Miner Metab | MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967 |
| Journal of Biomedical Materials Research | New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002 |
| Journal of Bone and Mineral Metabolism | MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967 |
| Scientific Reports | Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263 |
| International Journal of Molecular Sciences | Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730 |
| Pharmacology | Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457 |
| Experimental and Therapeutic Medicine | Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353 |
| American Journal of Translational Research | Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9 |
| The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro | |
| International Journal of Biomaterials | Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB091Ra01 | Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB091Ra01 | Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) | WB; IHC; ICC; IP. |
| MAB091Ra21 | Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) | WB; IHC; ICC; IP. |
| SEB091Ra | ELISA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB091Ra | CLIA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB091Ra | Magnetic Luminex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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