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SKU:PAB127Bo01

Polyclonal Antibody to Deoxyribonuclease I (DNASE1)

Polyclonal Antibody to Deoxyribonuclease I (DNASE1)

Regular price $400.00 USD
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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB127Bo01

Organism Species

Bos taurus; Bovine (Cattle).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against DNASE1. It has been selected for its ability to recognize DNASE1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
J Card Fail Low Circulating Levels of Mitochondrial and High Levels of Nuclear DNA Predict Mortality in Chronic Heart Failure Pubmed:27349571
Biochimie Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of … Pubmed: 32315661
Scientific Reports Biomarkers of neutrophil extracellular traps (NETs) and nitric oxide-(NO)-dependent oxidative stress in women who miscarried Pubmed: 32753622
J Am Heart Assoc DNase 1 Protects From Increased Thrombin Generation and Venous Thrombosis During Aging: Cross‐Sectional Study in Mice and Humans Pubmed:35023342
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPB127Bo01 Recombinant Deoxyribonuclease I (DNASE1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB127Bo01 Polyclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
MAB127Bo21 Monoclonal Antibody to Deoxyribonuclease I (DNASE1) WB; IHC; ICC; IP.
SEB127Bo ELISA Kit for Deoxyribonuclease I (DNASE1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB127Bo Magnetic Luminex Assay Kit for Deoxyribonuclease I (DNASE1) ,etc. Magnetic Luminex Assay for Antigen Detection.


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