Cloud-clone
SKU:PAB250Hu01
Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)
Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)
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;Immunohistochemistry: 5-20µg/mL;1:17-70
;Immunocytochemistry: 5-20µg/mL;1:17-70
;Optimal working dilutions must be determined by end user.
Product No.
PAB250Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB250Hu01-Recombinant Cluster Of Differentiation 73 (CD73)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Journal of Autoimmunity | The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation Pubmed:25640206 |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … 10.1016:j.talanta.2018.01.044 |
| Cancer Biomarkers | Expression and clinical significance of serum NT5E protein in patients with colorectal cancer Pubmed: 30932882 |
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