Cloud-clone
SKU:MAB317Hu22
Monoclonal Antibody to Perforin 1 (PRF1)
Monoclonal Antibody to Perforin 1 (PRF1)
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;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
MAB317Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
D1
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB317Hu01-Recombinant Perforin 1 (PRF1)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Diagnostic Microbiology and Infectious Disease | Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection Pubmed:26915636 |
| FASEB JOURNAL | The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis Pubmed: 30951375 |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790 |
| Mol Immunol | CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223 |
| INVESTIGATIONAL NEW DRUGS | Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342 |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237 |
| Nat Biomed Eng | Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy 34873307 |
| J Exp Med | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Pubmed:35061012 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB317Hu02 | Recombinant Perforin 1 (PRF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Hu01 | Active Perforin 1 (PRF1) | Cell culture; Activity Assays. |
| RPB317Hu01 | Recombinant Perforin 1 (PRF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB317Hu02 | Active Perforin 1 (PRF1) | Cell culture; Activity Assays. |
| PAB317Hu01 | Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| LAB317Hu81 | FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IF. |
| LAB317Hu71 | Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC. |
| MAB317Hu22 | Monoclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| MAB317Hu21 | Monoclonal Antibody to Perforin 1 (PRF1) | WB; IHC; ICC; IP. |
| SEB317Hu | ELISA Kit for Perforin 1 (PRF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB317Hu | Magnetic Luminex Assay Kit for Perforin 1 (PRF1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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