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SKU:PAB355Mu01

Polyclonal Antibody to 5-Lipoxygenase (5-LO)

Polyclonal Antibody to 5-Lipoxygenase (5-LO)

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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB355Mu01

Organism Species

Mus musculus (Mouse) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against 5-LO. It has been selected for its ability to recognize 5-LO in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Prostaglandins & Other Lipid Mediators Modulating effect of inositol hexaphosphate on arachidonic acid-dependent pathways in colon cancer cells. pubmed:28797636
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB355Mu02 Recombinant 5-Lipoxygenase (5-LO) Positive Control; Immunogen; SDS-PAGE; WB.
RPB355Mu01 Recombinant 5-Lipoxygenase (5-LO) Positive Control; Immunogen; SDS-PAGE; WB.
PAB355Mu01 Polyclonal Antibody to 5-Lipoxygenase (5-LO) WB; IHC; ICC; IP.
PAB355Mu02 Polyclonal Antibody to 5-Lipoxygenase (5-LO) WB; IHC; ICC; IP.
LAB355Mu71 Biotin-Linked Polyclonal Antibody to 5-Lipoxygenase (5-LO) WB; IHC; ICC.
SEB355Mu ELISA Kit for 5-Lipoxygenase (5-LO) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB355Mu Magnetic Luminex Assay Kit for 5-Lipoxygenase (5-LO) ,etc. Magnetic Luminex Assay for Antigen Detection


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