Cloud-clone
SKU:MAB357Hu22
Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
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;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
MAB357Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C3
Ig Isotype
IgG2a Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB357Hu01-Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Graefe's Archive for Clinical and Experimental Ophthalmology | Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome pubmed:27538909 |
| International Journal of Reproductive Medicine | Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis 33575317 |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
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