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SKU:PAB360Hu01
Polyclonal Antibody to Acid Sphingomyelinase (ASM)
Polyclonal Antibody to Acid Sphingomyelinase (ASM)
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;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAB360Hu01
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB360Hu01-Recombinant Acid Sphingomyelinase (ASM)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Mus musculus (Mouse), Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ASM. It has been selected for its ability to recognize ASM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Blood.? | Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126 |
| Journal of Clinical Lipidology | Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007 |
| Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696 |
| INFLAMMATION | Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756 |
| Indian J Ophthalmol | Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| EPB360Hu61 | Eukaryotic Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB360Hu01 | Recombinant Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB360Hu61 | Active Acid Sphingomyelinase (ASM) | Cell culture; Activity Assays. |
| PAB360Hu01 | Polyclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
| MAB360Hu22 | Monoclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
| SEB360Hu | ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB360Hu | Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB360Hu | Magnetic Luminex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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