Cloud-clone
SKU:MAB430Hu22
Monoclonal Antibody to Semaphorin 4D (SEMA4D)
Monoclonal Antibody to Semaphorin 4D (SEMA4D)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB430Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SEMA4D. It has been selected for its ability to recognize SEMA4D in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Expert Opin Ther Targets. | Circulating semaphorin-4D and plexin-B1 levels in postmenopausal women with low bone mass: the 3-month effect of zoledronic acid, denosumab or teriparatide treatment Pubmed:25395071 |
| Obesity (Silver Spring). | Genome-wide analysis identifies an african-specific variant in SEMA4D associated with body mass index. pubmed:28296344 |
| Clinical Lymphoma, Myeloma & Leukemia | A Revised Staging System for Waldenström's Macroglobulinemia 10.1016/j.clml.2017.07.146 |
| Clinical Lymphoma, Myeloma & Leukemia | Extramedullary Multiple Myeloma: Clinical Features and Prognosis in Argentina 10.1016/j.clml.2017.07.144 |
| Blood Cancer Journal | Semaphorin 4D correlates with increased bone resorption, hypercalcemia, and disease stage in newly diagnosed patients with multiple myeloma Pubmed:29748532 |
| Analytical Biochemistry | A high-sensitivity enzyme immunoassay for the quantification of soluble human semaphorin 4D in plasma Pubmed: 30879960 |
| Journal of Allergy and Clinical Immunology-In Practice | Pathological and therapeutic implications of eosinophil-derived semaphorin 4D in eosinophilic chronic rhinosinusitis Pubmed: 32035658 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB430Hu01 | Recombinant Semaphorin 4D (SEMA4D) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB430Hu01 | Polyclonal Antibody to Semaphorin 4D (SEMA4D) | WB; IHC; ICC; IP. |
| MAB430Hu22 | Monoclonal Antibody to Semaphorin 4D (SEMA4D) | WB; IHC; ICC; IP. |
| SEB430Hu | ELISA Kit for Semaphorin 4D (SEMA4D) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB430Hu | Magnetic Luminex Assay Kit for Semaphorin 4D (SEMA4D) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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