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SKU:MAB449Hu22
Monoclonal Antibody to Enolase 1 (ENO1)
Monoclonal Antibody to Enolase 1 (ENO1)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB449Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C2
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB449Hu02-Recombinant Enolase 1 (ENO1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against ENO1. It has been selected for its ability to recognize ENO1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Analytical Chemistry | Diagnostic detection of human lung cancer-associated antigen using a gold nanoparticle-based electrochemical immunosensor PubMed: 20557064 |
| Translational Research | Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385 |
| Journal of Proteomics | Putative salivary biomarkers useful to differentiate patients with fibromyalgia Pubmed:29654921 |
| BioMed Research International | The Diagnostic Accuracy of Combined Enolase/Cr, CA125, and CA19-9 in the Detection of Endometriosis Pubmed: 33062681 |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression 34376212 |
| Clin Proteomics | Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma Pubmed:35610567 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB449Hu03 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu02 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB449Hu01 | Recombinant Enolase 1 (ENO1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB449Hu02 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| PAB449Hu03 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| PAB449Hu01 | Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| LAB449Hu71 | Biotin-Linked Polyclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC. |
| MAB449Hu21 | Monoclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| MAB449Hu22 | Monoclonal Antibody to Enolase 1 (ENO1) | WB; IHC; ICC; IP. |
| SEB449Hu | ELISA Kit for Enolase 1 (ENO1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB449Hu | ELISA Kit for Anti-Enolase 1 Antibody (Anti-ENO1) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCB449Hu | CLIA Kit for Enolase 1 (ENO1) | Chemiluminescent immunoassay for Antigen Detection. |
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