Cloud-clone
SKU:MAB547Hu22
Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO)
Monoclonal Antibody to Indoleamine-2,3-Dioxygenase (IDO)
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;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
MAB547Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C13
Ig Isotype
IgG2b Kappa+ IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB547Hu01-Recombinant Indoleamine-2,3-Dioxygenase (IDO)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against IDO. It has been selected for its ability to recognize IDO in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Blood | CCL18 differentiates dendritic cells in tolerogenic cells able to prime regulatory T cells in healthy subjects Pubmed: 21803856 |
| Clinical Transplantation | Potential immunosuppressive function of plasma indoleamine 2,3-dioxygenase in patients with aGVHD after allo-HSCT Wiley: source |
| Indonesian Journal of Cancer Chemoprevention | Secretion of Indoleamine 2, 3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell Iregway: Source |
| PLoS ONE | CD40 Gene Silencing Reduces the Progression of Experimental Lupus Nephritis Modulating Local Milieu and Systemic Mechanisms PubMed: PMC3683035 |
| Hepatology | Human CD14+ CTLA-4+ regulatory dendritic cells suppress T-cell response by cytotoxic T-lymphocyte antigen-4-dependent IL-10 and indoleamine-2,3-dioxygenase production in hepatocellular carcinoma Pubmed: 23960017 |
| Free Radical Biology and Medicine | Indoleamine-2,3-dioxygenase elevated in tumor-initiating cells is suppressed by mitocans ScienceDirect: S0891584913006345 |
| Frontiers in Oncology | Cancer-Associated Fibroblasts from lung tumors maintain their immuno-suppressive abilities after high-dose irradiation Frontiersin:Source |
| Tissue Engineering Part A | Immunogenicity and escape mechanisms of allogeneic tendon-derived stem cells Pubmed:24813640 |
| Immunobiology. | The expression of dendritic cell subsets in severe chronic rhinosinusitis with nasal polyps is altered Pubmed:24947894 |
| Apoptosis | Chronic hepatitis C virus infection triggers spontaneous differential expression of biosignatures associated with T cell exhaustion and apoptosis signaling in peripheral blood mononucleocytes Pubmed:25577277 |
| Front Oncol | Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation PubMed: 26029659 |
| Acta Universitatis Tamperensis | Insights into the use of adipose stem cells for clinical cell therapy: Novel culturing conditions and characterization of multipotency and immunogenic properties Handle: 10024 |
| PLoS One | Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity via Interferon Beta and Indoleamine-2, 3-Dioxygenase pubmed:27695127 |
| Journal of Biomedical Nanotechnology | Exploring Amino Acid-Capped Nanoparticles for Selective Anti-Parasitic Action and Improved Host Biocompatibility Pubmed:29883557 |
| Biochemistry | Mechanisms of Leptin and Ghrelin Action on Maturation and Functions of Dendritic Cells |
| American Journal of Pathology | Cognitive Decline, Cerebral-Spleen Tryptophan Metabolism, Oxidative Stress, Cytokine Production, and Regulation of the Txnip Gene in 3xTg-AD Mice Pubmed: 30980800 |
| international immunopharmacology | Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression Pubmed: 31176083 |
| Stem Cell Reports | Richard Schäfer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Kuçi, 4 |
| International Journal of?Tryptophan?Research | Altered Indoleamine 2, 3-Dioxygenase Production and Its Association to Inflammatory Cytokines in Peripheral Blood Mononuclear Cells Culture of Type 2 Diabetes?¡ 33343199 |
| Stem Cell Reports | Richard Sch?fer,* Gabriele Spohn, Marco Bechtel, 2 Denisa Bojkova, 2 Patrick C. Baer, 3 Selim Ku?i, 4 |
| Transl Psychiatry | Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … 34802039 |
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