Cloud-clone
SKU:MAB622Hu22
Monoclonal Antibody to Surfactant Protein B (SP-B)
Monoclonal Antibody to Surfactant Protein B (SP-B)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAB622Hu22
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C7
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPB622Hu01-Recombinant Surfactant Protein B (SP-B)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SP-B. It has been selected for its ability to recognize SP-B in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Respiratory Physiology & Neurobiology | Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery PubMed: 21736957 |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594 |
| 19 | Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study PubMed: 20650982 |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896 |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032 |
| Cancer Cell. | Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models PubMed: PMC3406925 |
| Respir Physiol Neurobiol | Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project Pubmed: 23619193 |
| PLOS ONE | Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure Pubmed:25514679 |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source | |
| Int J Cardiol | Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study PubMed: 26310985 |
| Plos One | Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142 |
| PLoS One | The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877 |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052 |
| Frontiers in Aging Neuroscience | Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370 |
| Respiratory Physiology & Neurobiology | Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers pubmed:28467885 |
| Cellular Physiology and Biochemistry | Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production pubmed:28743125 |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126 |
| Respiratory research | Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649 |
| Respiratory Physiology & Neurobiology | Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan Pubmed:29548887 |
| Lung | Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934 |
| American Journal of Physiology Lung Cellular andMolecular Physiology | Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells Pubmed: 30211653 |
| Respiratory Research | Culture of human alveolar epithelial type II cells by sprouting Pubmed: 30340591 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB622Hu01 | Recombinant Surfactant Protein B (SP-B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB622Hu02 | Recombinant Surfactant Protein B (SP-B) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB622Hu01 | Polyclonal Antibody to Surfactant Protein B (SP-B) | WB; IHC; ICC; IP. |
| MAB622Hu22 | Monoclonal Antibody to Surfactant Protein B (SP-B) | WB; IHC; ICC; IP. |
| SEB622Hu | ELISA Kit for Surfactant Protein B (SP-B) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB622Hu | Magnetic Luminex Assay Kit for Surfactant Protein B (SP-B) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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