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SKU:MAB782Hu22

Monoclonal Antibody to Junctional Adhesion Molecule 1 (JAM1)

Monoclonal Antibody to Junctional Adhesion Molecule 1 (JAM1)

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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

MAB782Hu22

Organism Species

Homo sapiens (Human).

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

C11

Ig Isotype

IgG1 Kappa

Purification

Protein A + Protein G affinity chromatography

Label

None

Immunogen

RPB782Hu01-Recombinant Junctional Adhesion Molecule 1 (JAM1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a mouse monoclonal antibody raised against JAM1. It has been selected for its ability to recognize JAM1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Mediators Inflamm Early Detection of Junctional Adhesion Molecule-1 (JAM-1) in the Circulation after Experimental and Clinical Polytrauma PubMed: 26556956
JGH OPEN Cilostazol against 2, 4, 6‐trinitrobenzene sulfonic acid‐induced colitis: Effect on tight junction, inflammation, and apoptosis
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