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SKU:PAB827Ra01

Polyclonal Antibody to Netrin 1 (Ntn1)

Polyclonal Antibody to Netrin 1 (Ntn1)

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UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;1:120-500
Immunohistochemistry: 5-20µg/mL;1:12-50
Immunocytochemistry: 5-20µg/mL;1:12-50
Optimal working dilutions must be determined by end user.

Product No.

PAB827Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB827Ra01-Recombinant Netrin 1 (Ntn1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml



SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Ntn1. It has been selected for its ability to recognize Ntn1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:120-500
Immunohistochemistry: 5-20µg/mL;1:12-50
Immunocytochemistry: 5-20µg/mL;1:12-50
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Transplantation Proceedings Netrin-1: A Novel Universal Biomarker of Human Kidney Injury PubMed: 20620466
Biomarkers Plasma netrin-1 is a diagnostic biomarker of human cancers PubMed: PMC3143477
Journal of Maternal-Fetal and Neonatal Medicine Cord blood netrin-1 and -4 concentrations in term pregnancies with normal, restricted and increased fetal growth Informahealthcare: Source
4 Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity Pubmed:24584118
J Matern?Fetal?Neonatal Med.? Cord blood netrin-1 and-4 concentrations in term pregnancies with normal, restricted and increased fetal growth Pubmed:24716747
Renal failure. Urinary netrin-1 and KIM-1 as early biomarkers for septic acute kidney injury Pubmed:25154466
Stem Cell Research & Therapy Netrin-1 acts as a non-canonical angiogenic factor produced by human Wharton’s jelly mesenchymal stem cells (WJ-MSC) articles:10.1186
Oncotarget The effect of aspirin on circulating netrin-1 levels in humans is dependent on the inflammatory status of the vascular endothelium. pubmed:29156815
Frontiers in Cellular Neuroscience Neuroprotection Exerted by Netrin-1 and Kinesin Motor KIF1A in Secondary Brain Injury following Experimental Intracerebral Hemorrhage in Rats Pubmed:29375318
Scientific Reports Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor Pubmed:29662125
Family Practice and Palliative Care Relationship of serum netrin-1 levels with breast masses Doi: 10.22391/fppc.467700
JOURNAL OF BIOLOGICAL CHEMISTRY Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity Pubmed: 30910812
International Journal of Molecular Sciences Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus Pubmed: 30897795
Cancer Biomark Serum netrin-1 as a biomarker for colorectal cancer detection Pubmed: 32474463
Níveis Elevados de Netrina-1 e IL-1β em Mulheres Idosas com SCA: Pior Prognóstico no Acompanhamento de Dois Anos
Tuberk Toraks Association of plasma netrin-1 level with inflammatory balance and comorbidities in patients with acute exacerbation of COPD 33853303
Adv Healthc Mater Conductive Hydrogel Conduits with Growth Factor Gradients for Peripheral Nerve Repair in Diabetics with Non‐suture Tape Pubmed:35670309
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB827Ra01 Recombinant Netrin 1 (Ntn1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB827Ra01 Polyclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC; IP.
MAB827Ra21 Monoclonal Antibody to Netrin 1 (Ntn1) WB; IHC; ICC; IP.
SEB827Ra ELISA Kit for Netrin 1 (Ntn1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB827Ra Magnetic Luminex Assay Kit for Netrin 1 (Ntn1) ,etc. Magnetic Luminex Assay for Antigen Detection.

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