Cloud-clone
SKU:PAB831Ra01
Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)
Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAB831Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human), Mus musculus (Mouse)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html |
| Annals of the Rheumatic Diseases | Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672 |
| Catalog No. | Related products for research use of Rattus norvegicus (Rat) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB831Ra01 | Recombinant Hedgehog Homolog, Sonic (SHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB831Ra02 | Recombinant Hedgehog Homolog, Sonic (SHH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB831Ra01 | Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| PAB831Ra02 | Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| LAB831Ra71 | Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC. |
| MAB831Ra21 | Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) | WB; IHC; ICC; IP. |
| SEB831Ra | ELISA Kit for Hedgehog Homolog, Sonic (SHH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB831Ra | Magnetic Luminex Assay Kit for Hedgehog Homolog, Sonic (SHH) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB831Ra01 | ELISA Kit DIY Materials for Hedgehog Homolog, Sonic (SHH) | Main materials for "Do It (ELISA Kit) Yourself". |
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