Cloud-clone
SKU:PAB856Hu01
Polyclonal Antibody to Major Histocompatibility Complex Class I G (MHCG)
Polyclonal Antibody to Major Histocompatibility Complex Class I G (MHCG)
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Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.
Product No.
PAB856Hu01
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB856Hu01-Recombinant Major Histocompatibility Complex Class I G (MHCG)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; ICC; IHC-P; IHC-F; ELISA.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MHCG. It has been selected for its ability to recognize MHCG in immunohistochemical staining and western blotting.
USAGE
Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Lupus | Lack of association between HLA-G 14-bp polymorphism and systemic lupus erythematosus in a Han Chinese population SAGE: source |
| Exp Clin Transplant | Major Vascular Complications in Living-Donor Liver Transplant Recipients: Single Center Team Experience PubMed: 25894130 |
| International Immunopharmacology | Mesenchymal stem cells upregulate Treg cells via sHLA-G in SLE patients. pubmed:28129605 |
| Scientific Reports | Serum sHLA-G: Significant diagnostic biomarker with respect to therapy and immunosuppressive mediators in Head and Neck Squamous Cell Carcinoma Pubmed: 32123232 |
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